The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at a concentration of 0.1 - 1 µg/ml.
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1.0 - 10 µg/ml. Predicted molecular weight: 131 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
KCNT2 belongs to the potassium channel family, calcium-activated subfamily and contains one RCK N-terminal domain. KCNT2 is an outward rectifying potassium channel and produces rapidly activating outward rectifier K(+) currents. It is activated by high intracellular sodium and chloride levels. Channel activity is inhibited by ATP and by inhalation anesthetics, such as isoflurane. KCNT2 is inhibited upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. There are four named isoforms.