Anti-KCNT1/SLACK 抗体 [N3/26] (ab94578)
Key features and details
- Mouse monoclonal [N3/26] to KCNT1/SLACK
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-KCNT1/SLACK antibody [N3/26]
KCNT1/SLACK 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N3/26] to KCNT1/SLACK -
由来種
Mouse -
特異性
Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.
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アプリケーション
適用あり: ICC/IF, IHC-Fr, Flow Cyt, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Rat KCNT1/SLACK aa 1150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9Z258 -
ポジティブ・コントロール
- Flow Cyt: SH-SY5Y cells. WB: Rat brain lysate.
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特記事項
The clone number has been updated from S3-26 to N3/26, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N3/26 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000. Predicted molecular weight: 137 kDa.
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IHC-P |
1/1000.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 137 kDa. |
IHC-P
1/1000. |
ターゲット情報
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機能
Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). -
組織特異性
Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle. -
配列類似性
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.
Contains 1 RCK N-terminal domain. -
翻訳後修飾
Phosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 57582 Human
- Entrez Gene: 227632 Mouse
- Entrez Gene: 60444 Rat
- Omim: 608167 Human
- SwissProt: Q5JUK3 Human
- SwissProt: Q6ZPR4 Mouse
- SwissProt: Q9Z258 Rat
- Unigene: 104950 Human
see all -
別名
- bA100C15.2 antibody
- EIEE14 antibody
- ENFL5 antibody
see all
画像
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Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
HRP-conjugated sheet anti-mouse IgG
Predicted band size: 137 kDaMembrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.
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Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab94578 は 2 報の論文で使用されています。
- Li Y et al. A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats. Neurosci Bull 37:669-683 (2021). PubMed: 33779892
- Shore AN et al. Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy. Cell Rep 33:108303 (2020). PubMed: 33113364