Anti-KCNQ4 抗体 [N43/6] (ab84820)
Key features and details
- Mouse monoclonal [N43/6] to KCNQ4
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-KCNQ4 antibody [N43/6] -
製品の詳細
Mouse monoclonal [N43/6] to KCNQ4 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chimpanzee -
免疫原
Fusion protein corresponding to Human KCNQ4 aa 1-100.
Database link: P56696 -
ポジティブ・コントロール
- Lysate from transfected COS-1 cells transiently expressing KCNQ4.
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特記事項
The clone number has been updated from S43-6 to N43/6, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N43/6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84820の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
1/1000.
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IHC-P |
1/1000.
Fixation: Bouin’s Fixative |
|
WB |
1/1000. Predicted molecular weight: 77 kDa.
|
特記事項 |
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IHC-Fr
1/1000. |
IHC-P
1/1000. Fixation: Bouin’s Fixative |
WB
1/1000. Predicted molecular weight: 77 kDa. |
ターゲット情報
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機能
Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors. -
組織特異性
Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle. -
関連疾患
Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
配列類似性
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily. -
ドメイン
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded. -
細胞内局在
Basal cell membrane. Situated at the basal membrane of cochlear outer hair cells. - Information by UniProt
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参照データベース
- Entrez Gene: 456800 Chimpanzee
- Entrez Gene: 9132 Human
- Entrez Gene: 60613 Mouse
- Entrez Gene: 298496 Rat
- Omim: 603537 Human
- SwissProt: P56696 Human
- SwissProt: Q9JK97 Mouse
- SwissProt: Q9JK96 Rat
see all -
別名
- DFNA 2 antibody
- DFNA2 antibody
- KCNQ 4 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human hippocampus tissue labelling KCNQ4 with ab84820 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) (Green) at 1/50 dilution, for 1 hour at RT.
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Immunohistochemical analysis of Mouse brain tissue sections labeling KCNQ4 with ab84820 at 1/1000 dilution for 1 hour at room temperature.
Secondary Antibody: HRP/DAB Detection
System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature.
Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature
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All lanes : Anti-KCNQ4 antibody [N43/6] (ab84820) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Mixed cell lysates prepared from rat tissues Brain, Heart, Kidney, Liver, Lung, Pancreas, Skeletal Muscle, Spleen, Testes, Thymus, and H9C2 cells at 15 µg
Secondary
Lane 2 : Sheep Anti-Mouse IgG: HRP
Predicted band size: 77 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab84820 は 4 報の論文で使用されています。
- Wang Q et al. miR-153/KCNQ4 axis contributes to noise-induced hearing loss in a mouse model. J Physiol Sci 71:28 (2021). PubMed: 34479475
- Xiong H et al. Inhibition of Histone Methyltransferase G9a Attenuates Noise-Induced Cochlear Synaptopathy and Hearing Loss. J Assoc Res Otolaryngol N/A:N/A (2019). PubMed: 30710318
- Brueggemann LI et al. Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels. J Biol Chem 289:2099-111 (2014). WB, ICC/IF . PubMed: 24297175
- Udagawa T et al. Inwardly rectifying potassium channel Kir4.1 is localized at the calyx endings of vestibular afferents. Neuroscience 215:209-16 (2012). PubMed: 22546335