製品の概要

  • 製品名Anti-KCNQ2 antibody [S26A-23]
    KCNQ2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S26A-23] to KCNQ2
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC/IF, IP, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-59 of Human KCNQ2 (accession number O43526)

  • ポジティブ・コントロール
    • COS-1 cell lysate transiently expressing KCNQ2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Probably important in the regulation of neuronal excitability. Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. Muscarinic agonist oxotremorine-M strongly suppress KCNQ2/KCNQ3 current in cells in which cloned KCNQ2/KCNQ3 channels were coexpressed with M1 muscarinic receptors.
  • 組織特異性In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
  • 関連疾患Defects in KCNQ2 are the cause of benign familial neonatal seizures type 1 (BFNS1) [MIM:121200]. A disorder characterized by clusters of seizures occurring in the first days of life. Most patients have spontaneous remission by 12 months of age and show normal psychomotor development. Some rare cases manifest an atypical severe phenotype associated with epileptic encephalopathy and psychomotor retardation. The disorder is distinguished from benign familial infantile seizures by an earlier age at onset. In some patients, neonatal convulsions are followed later in life by myokymia, a benign condition characterized by spontaneous involuntary contractions of skeletal muscles fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Some patients may have isolated myokymia.
    Defects in KCNQ2 are the cause of epileptic encephalopathy early infantile type 7 (EIEE7) [MIM:613720]. EIEE7 is an autosomal dominant seizure disorder characterized by infantile onset of refractory seizures with resultant delayed neurologic development and persistent neurologic abnormalities.
  • 配列類似性Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily.
  • ドメインThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 翻訳後修飾In Xenopus oocytes KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminus region.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BFNC antibody
    • BFNS1 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • EIEE7 antibody
    • ENB 1 antibody
    • ENB1 antibody
    • HNSPC antibody
    • KCNA 11 antibody
    • KCNA11 antibody
    • KCNQ 2 antibody
    • Kcnq2 antibody
    • KCNQ2_HUMAN antibody
    • KQT like 2 antibody
    • KQT-like 2 antibody
    • KV7.2 antibody
    • KVEBN 1 antibody
    • KVEBN1 antibody
    • KvLQT 2 antibody
    • KvLQT2 antibody
    • Neuroblastoma specific potassium channel alpha subunit KvLQT2 antibody
    • Neuroblastoma specific potassium channel protein antibody
    • Neuroblastoma specific potassium channel subunit alpha antibody
    • Neuroblastoma specific potassium channel subunit alpha KvLQT2 antibody
    • Neuroblastoma-specific potassium channel subunit alpha KvLQT2 antibody
    • OTTHUMP00000031681 antibody
    • OTTHUMP00000031682 antibody
    • OTTHUMP00000031684 antibody
    • OTTHUMP00000031685 antibody
    • OTTHUMP00000031686 antibody
    • OTTHUMP00000031687 antibody
    • OTTHUMP00000031689 antibody
    • Potassium voltage gated channel KQT like protein 2 antibody
    • Potassium voltage gated channel KQT like subfamily member 2 antibody
    • Potassium voltage gated channel subfamily KQT member 2 antibody
    • Potassium voltage-gated channel subfamily KQT member 2 antibody
    • Voltage gated potassium channel subunit Kv7.2 antibody
    • Voltage-gated potassium channel subunit Kv7.2 antibody
    see all

Anti-KCNQ2 antibody [S26A-23] 画像

  • Overlay histogram showing SH-SH5Y cells stained with ab84812 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84812, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-KCNQ2 antibody [S26A-23] (ab84812) at 10 µg/ml

    Lane 1 : CHO cell lysate
    Lane 2 : HEK cell lysate


    Predicted band size : 95 kDa
  • ab84812 staining KCNQ2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • ab84812 staining KCNQ2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

Anti-KCNQ2 antibody [S26A-23] (ab84812) 使用論文

This product has been referenced in:
  • Nieto-Gonzalez JL & Jensen K BDNF Depresses Excitability of Parvalbumin-Positive Interneurons through an M-Like Current in Rat Dentate Gyrus. PLoS One 8:e67318 (2013). Read more (PubMed: 23840662) »

See 1 Publication for this product

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