製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KCNN4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human KCNN4.

    (Peptide available as ab87266.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates: Skin Placenta

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能
    Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
  • 組織特異性
    Widely expressed in non-excitable tissues.
  • 配列類似性
    Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
  • 翻訳後修飾
    Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DHS2 antibody
    • hIKCa1 antibody
    • hKCa4 antibody
    • hSK4 antibody
    • IK antibody
    • IK1 antibody
    • IKCa1 antibody
    • Intermediate conductance calcium activated potassium channel protein 4 antibody
    • Intermediate conductance calcium-activated potassium channel protein 4 antibody
    • KCa3.1 antibody
    • KCa4 antibody
    • KCNN 4 antibody
    • Kcnn4 antibody
    • KCNN4_HUMAN antibody
    • Potassium calcium activated channel subfamily N member 4 antibody
    • Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 antibody
    • Putative Gardos channel antibody
    • SK4 antibody
    • SKCa 4 antibody
    • SKCa4 antibody
    see all

画像

  • All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Morales P  et al. Contribution of the KCa3.1 channel-calmodulin interactions to the regulation of the KCa3.1 gating process. J Gen Physiol 142:37-60 (2013). WB . Read more (PubMed: 23797421) »

See 1 Publication for this product

レビューと Q&A

Thank you for your patience. Here is the homology between human and mouse for the following antibodies (based on the alignment of their immunogen sequences) ab55452: homology = 94% - crossreactivity very likely, but not tested ab57662: homolog...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (Right Coronary Artery & Lung)
Loading amount
20 µg
Specification
Right Coronary Artery & Lung
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20% Tris Ready Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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投稿 Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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