Anti-KCNN4 抗体 (ab75956)
Key features and details
- Rabbit polyclonal to KCNN4
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-KCNN4 antibody
KCNN4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KCNN4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Pig -
免疫原
Synthetic peptide corresponding to Human KCNN4 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87266) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following human tissue lysates: Skin Placenta
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75956の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. -
組織特異性
Widely expressed in non-excitable tissues. -
配列類似性
Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily. -
翻訳後修飾
Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3783 Human
- Entrez Gene: 397081 Pig
- Omim: 602754 Human
- SwissProt: O15554 Human
- Unigene: 10082 Human
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別名
- DHS2 antibody
- hIKCa1 antibody
- hKCa4 antibody
see all
画像
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All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
The 50 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to KCNN4.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab75956 は 3 報の論文で使用されています。
- Sun WT et al. Homocysteine alters vasoreactivity of human internal mammary artery by affecting the KCa channel family. Ann Transl Med 9:625 (2021). PubMed: 33987323
- Ma W et al. Successful Application of a Galanin-Coated Scaffold for Periodontal Regeneration. J Dent Res 100:1144-1152 (2021). PubMed: 34328037
- Morales P et al. Contribution of the KCa3.1 channel-calmodulin interactions to the regulation of the KCa3.1 gating process. J Gen Physiol 142:37-60 (2013). WB . PubMed: 23797421