The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily.
Predicted band size : 82 kDa ab28631 at 2µg/ml used in Western blot. Negative control: mouse kidney lysate, positive sample: mouse whole brain lysate. The secondary antibody was goat anti-rabbit and used at 1/10,000 dilution.