製品の概要

  • 製品名Anti-KCNN2 antibody
    KCNN2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to KCNN2
  • 特異性Recognizes both Human isoforms (NP_067627.2 and NP_740721.1)
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan
  • 免疫原

    Synthetic peptide: C-

    ESYDKHVTYNAER

    , corresponding to internal amino acids 546 - 557 of Human KCNN2 (NP_067627.2) and 197 - 209 of Human KCNN2 isoform b (NP_740721.1).

  • ポジティブ・コントロール
    • Liver lysate Human thyroid

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).Can be blocked with KCNN2 peptide (ab186680).

ターゲット情報

Anti-KCNN2 antibody 画像

  • Anti-KCNN2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 64, 26 kDa
    Observed band size : 23,70 kDa (why is the actual band size different from the predicted?)
  • ab99457 at 3.8 µg/ml staining KCNN2 in Human thyroid by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody was revealed by AP-staining.

Anti-KCNN2 antibody (ab99457) 使用論文

ab99457 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spinal cord)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, pH6
Permeabilization Yes - 0.1% Tween-20
Specification spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mrs. Teresa Garrett

Verified customer

投稿 Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"