製品の概要

  • 製品名Anti-KCNN2 antibody
    KCNN2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KCNN2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 72-121 (KNAAANVLRE TWLIYKNTKL VKKIDHAKVR KHQRKFLQAI HQLRSVKMEQ) of Human KCNN2 isoform b (NP_740721).

  • ポジティブ・コントロール
    • Human fetal muscle lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-KCNN2 antibody 画像



  • Predicted band size : 26 kDa
    Western blot analysis of Human fetal muscle tissue lysate labeling KCNN2 with ab85401 at 1.0µg/ml.


  • Predicted band size : 26 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling KCNN2 with ab85401 at 1.0µg/ml.


  • Predicted band size : 26 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling KCNN2 with ab85401 at 1.0µg/ml.
  • Anti-KCNN2 antibody (ab85401) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    12% gel
  • ab85401 staining KCNN2 in Rhesus macaque spinal cord by ICC/IF (Immunocytochemistry/immunofluorescence).  Samples were incubated with primary antibody (1/300). An Alexa Fluor®488-conjugated Donkey anti-rabbit (1/500) was used as the secondary antibody.

Anti-KCNN2 antibody (ab85401) 使用論文

This product has been referenced in:
  • Chakroborty S  et al. Early presynaptic and postsynaptic calcium signaling abnormalities mask underlying synaptic depression in presymptomatic Alzheimer's disease mice. J Neurosci 32:8341-53 (2012). WB ; Mouse . Read more (PubMed: 22699914) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab85401.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"