製品の概要

  • 製品名Anti-KCNK9 antibody
  • 製品の詳細
    Rabbit polyclonal to KCNK9
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 36-85 (

    REEEKLKAEEIRIKGKYNISSEDYRQLELVILQSEPHRAGVQWKFAGSFY

    ) of human KCNK9 (NP_057685)

  • ポジティブ・コントロール
    • HepG2 Cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性KCNK9 or TASK-3 (TWIK-related Acid sensitive K+ channel) is a member of the potassium channel family of proteins that contain two-pore domain and four transmembrane domains. These channels are characterized as leak K+ channels that are sensitive to changes in the extracellular pH. The physiological functions of TASK channels are largely unknown; it has been proposed that they may be involved in the regulation of breathing, aldosterone secretion and anesthetic-mediated neuronal activity. They were found to act in neurons' membrane potential and in resting K+ currents. KCNK9 gene has been localized to the chromosomal region 8q24. The protein is expressed at high levels mainly in the brain and at low levels in other tissues. In contrast to normal tissues, it was found that KCNK9 is amplified in some human carcinomas such as breast, lung, colon and metastatic prostate. In 10% of breast cancer patients this gene is amplified, and in 44% the protein is over expressed. Monoclonal antibodies to KNCK9 are an important tool for studying the potassium channel family of proteins in different tissues.
  • 細胞内局在Plasma membrane
  • 参照データベース
  • 別名
    • Acid sensitive potassium channel protein TASK 3 antibody
    • K2p9.1 antibody
    • KT3.2 antibody
    • MGC138268 antibody
    • MGC138270 antibody
    • Potassium channel TASK3 antibody
    • Potassium channel, subfamily K, member 9 (TASK 3) antibody
    • Potassium channel, subfamily K, member 9 antibody
    • TASK 3 antibody
    • TWIK related acid sensitive K+ channel 3 antibody
    see all

Anti-KCNK9 antibody 画像



  • Predicted band size : 42 kDa
    Western blot analysis of Human fetal brain tissue lysate labeling KCNK9 with ab85289 at 1.0µg/ml.
  • Anti-KCNK9 antibody (ab85289) at 1 µg/ml + HepG2 Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1: 50,000

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • ab85289 staining KCNK9 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling KCNNK9 with ab85289 at 5.0ug/ml.

Anti-KCNK9 antibody (ab85289) 使用論文

ab85289 has not yet been referenced specifically in any publications.

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