製品の概要

  • 製品名
    Anti-KCNK10 antibody
  • 製品の詳細
    Rabbit polyclonal to KCNK10
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    A region within synthetic peptide: HGQGASEDNI INKFGSTSRL TKRKNKDLKK TLPEDVQKIY KTFRNYSLDE, corresponding to C terminal amino acids 434-483 of Human KCNK10

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.4 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

ターゲット情報

  • 機能
    Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents. Activated by arachidonic acid and other naturally occurring unsaturated free fatty acids.
  • 組織特異性
    Abundantly expressed in pancreas and kidney and to a lower level in brain, testis, colon, and small intestine. Isoform b is strongly expressed in kidney (primarily in the proximal tubule) and pancreas, whereas isoform c is abundantly expressed in brain.
  • 配列類似性
    Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2P domain potassium channel TREK2 antibody
    • K2p10.1 antibody
    • KCNK10 antibody
    • KCNKA_HUMAN antibody
    • Outward rectifying potassium channel protein TREK 2 antibody
    • Outward rectifying potassium channel protein TREK-2 antibody
    • Potassium channel subfamily K member 10 antibody
    • Potassium channel TREK 2 antibody
    • TREK 2 antibody
    • TREK 2 K antibody
    • TREK 2 K(+) channel subunit antibody
    • TREK-2 K(+) channel subunit antibody
    • TREK2 antibody
    • TWIK related K+ channel 2 antibody
    see all

画像

  • Anti-KCNK10 antibody (ab48868) at 1.4 µg/ml (in 5% skimmed milk/PBS) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab48868 has not yet been referenced specifically in any publications.

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