製品の概要

  • 製品名Anti-KCNJ5 antibody
    KCNJ5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KCNJ5
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KCNJ5 .

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Testis tissue lysate as well as the following whole cell lysates: A489; MCF7; A549. It also gave a positive signal in FFPE human pancreas tissue sections

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
  • 組織特異性Islets, exocrine pancreas and heart.
  • 関連疾患Defects in KCNJ5 are the cause of long QT syndrome type 13 (LQT13) [MIM:613485]. It is a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • 配列類似性Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac ATP sensitive potassium channel antibody
    • Cardiac inward rectifier antibody
    • CIR antibody
    • G protein activated inward rectifier potassium channel 4 antibody
    • G protein-activated inward rectifier potassium channel 4 antibody
    • GIRK 4 antibody
    • GIRK-4 antibody
    • GIRK4 antibody
    • Heart KATP channel antibody
    • Inward rectifier K(+) channel Kir3.4 antibody
    • Inward rectifier K+ channel KIR3.4 antibody
    • inwardly rectifying subfamily J member 5 antibody
    • IRK-4 antibody
    • IRK5_HUMAN antibody
    • KATP 1 antibody
    • KATP-1 antibody
    • KATP1 antibody
    • KCNJ 5 antibody
    • Kcnj5 antibody
    • KIR 3.4 antibody
    • KIR3.4 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 5 antibody
    • Potassium inwardly rectifying channel J5 antibody
    • Potassium inwardly rectifying channel subfamily J member 5 antibody
    see all

Anti-KCNJ5 antibody 画像

  • All lanes : Anti-KCNJ5 antibody (ab113699) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • IHC image of KCNJ5 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113699, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KCNJ5 antibody (ab113699) 使用論文

ab113699 has not yet been referenced specifically in any publications.

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