製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KCNIP1
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: MGAVMGTFSS LQTKQRRPSK DKIEDELEMT MVCHRPEGLE QLEAQTNFTK, corresponding to a region within N terminal amino acids 1-50 of Human KCNIP1

  • ポジティブ・コントロール
    • Jurkat cells. Brain.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

WB Use a concentration of 1.35 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface.
  • 組織特異性
    Isoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus.
  • 配列類似性
    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • 細胞内局在
    Cell membrane. Cytoplasm. Cell projection, dendrite.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are 3 isoforms produced by alternative splicing.
  • 別名
    • A type potassium channel modulatory protein 1 antibody
    • A-type potassium channel modulatory protein 1 antibody
    • KChIP1 antibody
    • KCIP1_HUMAN antibody
    • Kcnip1 antibody
    • Kv channel interacting protein 1 antibody
    • Kv channel-interacting protein 1 antibody
    • MGC9 antibody
    • MGC95 antibody
    • Potassium channel interacting protein 1 antibody
    • Potassium channel-interacting protein 1 antibody
    • VABP antibody
    • Vesicle APC binding protein antibody
    • Vesicle APC-binding protein antibody
    see all

画像

  • Anti-KCNIP1 antibody (ab66628) at 1.35 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling KCNIP1 with ab66628 at 4-8µg/ml. Arrows indicate positively labelled neural cells. Magnification: 400X.

参考文献

ab66628 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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