製品の概要

  • 製品名Anti-KCNH5 antibody
  • 製品の詳細
    Rabbit polyclonal to KCNH5
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide derived from a region between amino acids 156-205 of Human KCNH5. Note: The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HepG2 cell lysate and human brain tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/312500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 112 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. The KCNH5 gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. KCNH5 is not expressed in differentiating myoblasts.
  • 細胞内局在Cell Membrane: multi-pass membrane protein.
  • 参照データベース
  • 別名
    • EAG2 antibody
    • Ether a go go potassium channel 2 antibody
    • H EAG2 antibody
    • HGNC:6254 antibody
    • Kv10.2 antibody
    • potassium voltage gated channel subfamily H (eag-related) member 5 antibody
    • Voltage gated potassium channel subunit Kv10.2 antibody
    see all

Anti-KCNH5 antibody 画像

  • Anti-KCNH5 antibody (ab32975) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 112 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling KCNH5 at 4-8µg/ml. Neural cells with positive label are indicated by arrows.

Anti-KCNH5 antibody (ab32975) 使用論文

This product has been referenced in:
  • Huang X  et al. Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics. Genes Dev 26:1780-96 (2012). Read more (PubMed: 22855790) »
  • Macaulay EC  et al. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm Genome 22:722-35 (2011). IHC-P ; Human . Read more (PubMed: 21874386) »

See all 2 Publications for this product

Product Wall

Vielen Dank für diese Rückmeldung. Es tut mir wirklich sehr leid, dass Sie keine zufriedenstellenden Ergebnisse erhalten haben! Zu den KCNH5-Antikörpern: Es ist in der Tat sehr einfach, in den 'Additional Notes' eine kurze Notiz zu den erzielten Ergebn...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"