製品の概要

  • 製品名Anti-KChIP2 antibody
    KChIP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KChIP2
  • 特異性Detects KChIP 2 b and c from rat and rabbit samples as well as recombinant rat KChIP 2c. This antibody does not detect KChIP 2a.
  • アプリケーション適用あり: IHC-P, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Dog, Non Human Primates
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide corresponding to Human KChIP2 aa 6-24.
    Sequence:

    RKESLSDSRDLDGSYDQLT


    (Peptide available as ab4991)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Voltage-gated potassium (Kv) channels are key components of the potassium currents in the heart and central nervous system. In the heart, Kv4 channels are involved in the repolarization phase of the action potential. In the brain however, these channels prevent reverse-propagation of action potentials. Found associated with Kv4 channels are a group of calcium-binding proteins termed KChIPs (Kv channel interacting proteins). KChIPs are small molecular weight proteins that bind to the cytoplasmic amino termini of Kv4 alpha-subunits and help to modulate its function. There are currently three known KChIPs; KChIP 1 expressed in the brain; KChIP 2 expressed in the brain, heart, and lung, and has three isoforms, a, b, and c; and KChIP 3, also known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator) which is expressed in the brain and testis.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Can be blocked with KChIP2 peptide (ab4991).

By Western blot, this antibody detects ~33 and 26 kDa proteins representing KChIP2b and c, respectively, from rat ventricular myocyte whole cell lysate.

ターゲット情報

  • 機能Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart.
  • 組織特異性Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein endothelial cells. Not expressed in fetal heart.
  • 配列類似性Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • 翻訳後修飾Palmitoylated. Palmitoylation enhances association with the plasma membrane.
  • 細胞内局在Cell membrane and Cell membrane. Detected on lipid rafts (By similarity).
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 9 isoforms produced by alternative splicing.
  • 別名
    • A type potassium channel modulatory protein 2 antibody
    • A-type potassium channel modulatory protein 2 antibody
    • Cardiac voltage gated potassium channel modulatory subunit antibody
    • Cardiac voltage-gated potassium channel modulatory subunit antibody
    • DKFZp566L1246 antibody
    • KChIP 2 antibody
    • KChIP2 antibody
    • KCIP2_HUMAN antibody
    • KCNIP 2 antibody
    • Kcnip2 antibody
    • Kv channel interacting protein 2 antibody
    • Kv channel-interacting protein 2 antibody
    • MGC17241 antibody
    • Potassium channel interacting protein 2 antibody
    • Potassium channel-interacting protein 2 antibody
    see all

Anti-KChIP2 antibody (ab3473) 使用論文

ab3473 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (heart)
Loading amount 40 µg
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Apr 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"