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Synthetic peptide: AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG, corresponding to a region within C terminal amino acids 541-590 of Human KBTBD10
Our Abpromise guarantee covers the use of ab66605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24268659|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemistry (Frozen sections) analysis of human skeletal muscle tissue from control and nemaline myopathy affected individuals labelling KBTBD10 with ab66605. Costaining with sarcomeric markers in longitudinal planes showed that KLHL41 staining predominated over the I-bands of the sarcomere and at perinuclear regions. Scale bars represent 50 μm.