製品の概要

  • 製品名Anti-KATNA1 antibody [EPR5071]
    KATNA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5071] to KATNA1
  • アプリケーション適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to residues from the C terminus of Human KATNA1.

  • ポジティブ・コントロール
    • WB: HeLa and HepG2 cell lysates. ICC/IF: HeLa cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab111881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth.
    • 配列類似性Belongs to the AAA ATPase family.
    • ドメインThe N-terminus is sufficient for interaction with microtubules, although high affinity binding requires an intact C-terminal domain and ATP, which promotes oligomerization.
    • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Predominantly cytoplasmic. Also localized to the interphase centrosome. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Katanin p60 ATPase containing subunit A1 antibody
      • Katanin p60 ATPase-containing subunit A1 antibody
      • Katanin p60 subunit A1 antibody
      • KATNA1 antibody
      • KTNA1_HUMAN antibody
      • OTTHUMP00000017392 antibody
      • OTTHUMP00000042860 antibody
      • p60 katanin antibody
      see all

    Anti-KATNA1 antibody [EPR5071] 画像

    • All lanes : Anti-KATNA1 antibody [EPR5071] (ab111881) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 56 kDa
    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KATNA1 with ab111881 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing staining on the centrosome of Hela cells.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab111881 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    Anti-KATNA1 antibody [EPR5071] (ab111881) 使用論文

    ab111881 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"