Anti-KAT8 / MYST1 / MOF 抗体 (ab72056)
Key features and details
- Rabbit polyclonal to KAT8 / MYST1 / MOF
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KAT8 / MYST1 / MOF antibody
KAT8 / MYST1 / MOF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAT8 / MYST1 / MOF -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide corresponding to Human KAT8/ MYST1/ MOF aa 1-100 (N terminal).
Database link: Q9H7Z6 -
ポジティブ・コントロール
- Whole cell lysate from HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72056の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. -
配列類似性
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
Contains 1 chromo domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 84148 Human
- Omim: 609912 Human
- SwissProt: Q9H7Z6 Human
- Unigene: 533803 Human
-
別名
- Histone acetyltransferase KAT8 antibody
- Histone acetyltransferase MYST1 antibody
- hMOF antibody
see all
画像
-
All lanes : Anti-KAT8 / MYST1 / MOF antibody (ab72056) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 19 kDa, 28 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KAT8 / MYST1 / MOF with ab72056 at 1/1000 (1µg/ml). Detection: DAB.
-
Detection of Human KAT8 / MYST1 / MOF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72056 at 3 µg/mg lysate. Subsequent WB detection was done using ab72056 at 1 µg/ml.
-
IHC image of ab72056 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72056, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab72056 は 3 報の論文で使用されています。
- Guo R et al. The Histone Acetyltransferase MOF Regulates SIRT1 Expression to Suppress Renal Cell Carcinoma Progression. Front Oncol 12:842967 (2022). PubMed: 35252011
- Li L & Wang Y Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair. J Biol Chem 292:11951-11959 (2017). PubMed: 28546430
- Liu Y et al. MDM2-MOF-H4K16ac axis contributes to tumorigenesis induced by Notch. FEBS J 281:3315-24 (2014). PubMed: 24898892