製品の概要

  • 製品名Anti-KAT7 / Hbo1 / MYST2 antibody
    KAT7 / Hbo1 / MYST2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KAT7 / Hbo1 / MYST2
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from an internal region of human KAT7/ Hbo1/ MYST2

  • ポジティブ・コントロール
    • HUVEC cells, extracts from Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ELISA 1/20000.
ICC/IF 1/100 - 1/500.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.
  • 組織特異性Ubiquitously expressed, with highest levels in testis.
  • 配列類似性Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
  • ドメインThe C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
    The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hbo 1 antibody
    • HBO1 antibody
    • HBOa antibody
    • Histone acetyltransferase binding to hORC1 antibody
    • Histone acetyltransferase binding to ORC1 antibody
    • Histone acetyltransferase KAT7 antibody
    • Histone acetyltransferase MYST2 antibody
    • K(lysine) acetyltransferase 7 antibody
    • KAT 7 antibody
    • KAT7 antibody
    • Lysine acetyltransferase 7 antibody
    • MOZ antibody
    • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
    • MYST 2 antibody
    • MYST histone acetyltransferase 2 antibody
    • MYST protein 2 antibody
    • MYST-2 antibody
    • MYST2 antibody
    • MYST2_HUMAN antibody
    • SAS 2 antibody
    • SAS2 and TIP60 protein 2 antibody
    • TIP60 protein 2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC7 antibody
    see all

Anti-KAT7 / Hbo1 / MYST2 antibody 画像

  • All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 71 kDa
    Observed band size : 71 kDa
  • HUVEC cells with ab70183 at 1/500 dilution, with and without the immunising peptide.
  • IHC image of ab70183 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • KAT7 / Hbo1 / MYST2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to KAT7 / Hbo1 / MYST2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab70183.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 71kDa; KAT7 / Hbo1 / MYST2; non specific bands - 80kDa: We are unsure as to the identity of this extra band.

Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183) 使用論文

This product has been referenced in:
  • Zou C  et al. SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition complex (HBO1) ubiquitin-proteasomal degradation to regulate cell proliferation. J Biol Chem 288:6306-16 (2013). WB ; Mouse . Read more (PubMed: 23319590) »
  • Lalonde ME  et al. Exchange of associated factors directs a switch in HBO1 acetyltransferase histone tail specificity. Genes Dev 27:2009-24 (2013). Read more (PubMed: 24065767) »

See all 3 Publications for this product

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