Anti-KAT7 / Hbo1 / MYST2 抗体 (ab70183)
Key features and details
- Rabbit polyclonal to KAT7 / Hbo1 / MYST2
- Suitable for: ELISA, IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-KAT7 / Hbo1 / MYST2 antibody
KAT7 / Hbo1 / MYST2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAT7 / Hbo1 / MYST2 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-P, WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70183の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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ICC/IF |
1/100 - 1/500.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
ICC/IF
1/100 - 1/500. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription. -
組織特異性
Ubiquitously expressed, with highest levels in testis. -
配列類似性
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger. -
ドメイン
The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 11143 Human
- Entrez Gene: 217127 Mouse
- Entrez Gene: 303470 Rat
- Omim: 609880 Human
- SwissProt: O95251 Human
- SwissProt: Q5SVQ0 Mouse
- SwissProt: Q810T5 Rat
- Unigene: 21907 Human
see all -
別名
- Hbo 1 antibody
- HBO1 antibody
- HBOa antibody
see all
画像
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All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa -
HUVEC cells with ab70183 at 1/500 dilution, with and without the immunising peptide.
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IHC image of ab70183 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab70183 は 13 報の論文で使用されています。
- Xie M et al. Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis. Exp Mol Med 53:572-590 (2021). PubMed: 33811247
- MacPherson L et al. HBO1 is required for the maintenance of leukaemia stem cells. Nature 577:266-270 (2020). PubMed: 31827282
- Khan O et al. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion. Nature 571:211-218 (2019). PubMed: 31207603
- Long C et al. LPS promotes HBO1 stability via USP25 to modulate inflammatory gene transcription in THP-1 cells. Biochim Biophys Acta Gene Regul Mech 1861:773-782 (2018). PubMed: 30745998
- Yan MS et al. Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation. J Biol Chem 293:4381-4402 (2018). PubMed: 29414790