特異性ab79270 recognizes KAT3A as well as CBP. KAT3A and CBP differ by three noncontiguous residues within the 21 amino acid epitope region, a
difference that does not detectably affect the reactivity of the antibody.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100 for 20-30 minutes at room temperature depending on detection system.
Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
関連疾患Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription. Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
翻訳後修飾Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response. Phosphorylated upon DNA damage, probably by ATM or ATR. Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
細胞内局在Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.