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Immunogen was a synthetic peptide, which represented a portion of human CREB binding protein encoded within exon 33 (LocusLink ID 1387)(between residues 2392 and the c-terminus (residue 2442) according to Swiss-Prot entry Q92793).
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
Our Abpromise guarantee covers the use of ab10490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 1-4 µg/mg of lysate.|
|ChIP||Use 2-4µg for 106 cells.|
|WB||1/1000 - 1/25000. Predicted molecular weight: 265 kDa.Can be blocked with Human KAT3A / CBP peptide (ab4916).|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab10490 immunoprecipitation of CREB Binding Protein.
Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: ab10490 used at 20
Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs. 2–4 µg of ab10490 and 1-2x106 cells were used in each ChIP experiment.
Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL
ab10490 has not yet been referenced specifically in any publications.