Anti-KAT2B / PCAF 抗体 - ChIP Grade (ab12188)
製品の概要
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製品名Anti-KAT2B / PCAF antibody - ChIP Grade
KAT2B / PCAF 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to KAT2B / PCAF - ChIP Grade
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由来種Rabbit
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アプリケーション適用あり: IHC-P, ChIP, WB, IP, ICC/IFmore details
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種交差性交差種: Mouse, Dog, Human
交差が予測される動物種: Chicken
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免疫原
Synthetic peptide:
EKFFFSKIKEAGLIDK
(with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF. -
ポジティブ･コントロール
- Immunoblotting: Whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF. Immunoprecipitation: RIPA lysate, human acute T cell leukemia Jurkat cells.
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特記事項If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab12188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ChIP | Use at an assay dependent concentration. PubMed: 16829531Use at an assay dependent dilution. | |
| WB | 1/500. Predicted molecular weight: 93 kDa. 1/500 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 93 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide. | |
| IP | Use 5µg for 107 cells. 5-10µg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 107 human acute T cell leukemia Jurkat cells. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20410122 |
ターゲット情報
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機能Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
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組織特異性Ubiquitously expressed but most abundant in heart and skeletal muscle.
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配列類似性Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
ドメインThe bromodomain mediates binding to HIV-1 Tat.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 428441 Chicken
- Entrez Gene: 8850 Human
- Entrez Gene: 18519 Mouse
- Omim: 602303 Human
- SwissProt: Q92831 Human
- SwissProt: Q9JHD1 Mouse
- Unigene: 533055 Human
- Unigene: 255025 Mouse
see all -
別名
- CAF antibody
- CREBBP associated factor antibody
- GCN5 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)This image is courtesy of an anonymous Abreviewab12188 at 2ug/ml staining human thymus tissue sections by IHC-P. The tissue was formaldehyde fixed and then a heat mediated antigen retrieval step was carried out. The antibody was incubated with the tissue for 30 minutes. An HRP conjugated goat antibody was used as the secondary. -
ChIP - Anti-KAT2B / PCAF antibody - ChIP Grade (ab12188)This image is courtesy of an anonymous AbreviewChromatin was prepared from mouse skin epidermis whole tissue lysate. Cells were fixed with 1% fromaldehyde for 10 minutes. TPA treated samples were incubated with rabbit IgG, HDAC7 antibody or ab12188 (diluted 1/800) for 12 hours at 4°C and the immunoprecipitated DNA was amplified using primers flanking the proximal promoter region and quantified by semiquantitative PCR. Rabbit IgG and another unrelated antibody immunoprecipitated sample did not produce significant amplification.
プロトコール
参考文献
This product has been referenced in:
- Jeitany M et al. Opposite effects of GCN5 and PCAF knockdowns on the alternative mechanism of telomere maintenance. Oncotarget 8:26269-26280 (2017). Read more (PubMed: 28412741) »
- Perearnau A et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res 45:5086-5099 (2017). Read more (PubMed: 28158851) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"