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Products:Neuroscience >> Neurology process >> Circadian Rhythm
Overview
Product name
Anti-KAT13D / CLOCK antibody - ChIP Grade
KAT13D / CLOCK 抗体 (9件) 一覧
Description
Rabbit polyclonal to KAT13D / CLOCK - ChIP Grade
Tested applications
IHC-P, IHC-Fr, WB, ChIP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Human
Immunogen
Synthetic peptide: S(11)SIVDRDDSSIFDGLVEEDD(30)C. Immunizi ng peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK.
SSIVDRDDSS IFDGLVEEDD -C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Blood >> Blood Pressure regulation
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Circadian Rhythm
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (Immunohistochemical staining of CLOCK in hamster brain results in the staining of the superchiasmatic nucleus.)
IHC-Fr
IHC-Fr: 1/1000IHC-Fr: 1/1000
WB: 1/200Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).(By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. )
ChIP: Use at an assay dependent dilution. (PubMed: 20956306)
ICC/IF: Use at an assay dependent concentration. (PubMed: 22028779)
Target
Function
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.
Tissue specificity
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Post-translational
modifications
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.
Cellular localization
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.
Target information above from: UniProt accessionO15516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
Database links
- Entrez Gene: 9575 Human
- Entrez Gene: 12753 Mouse
- Entrez Gene: 60447 Rat
- Omim: 601851 Human
- SwissProt: O15516 Human
- SwissProt: O08785 Mouse
- SwissProt: Q9WVS9 Rat
- Unigene: 436975 Human
see all
Anti-KAT13D / CLOCK antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAT13D / CLOCK antibody (ab3517)
ab3517 staining KAT13D/CLOCK in Mouse skeletal muscle tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded tissue sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer pH6. Samples were incubated with primary antibody (1/400 in PBS) for 12 hours at 4°C. Undiluted ab64256 was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - KAT13D / CLOCK antibody (ab3517)
All lanes : Anti-KAT13D / CLOCK antibody - ChIP Grade (ab3517) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Additional bands at : 105 kDa (possible post-translational modification),38 kDa,45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
KAT13D / CLOCK contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern observed at a higher molecular weight than predicted.
References for Anti-KAT13D / CLOCK antibody - ChIP Grade (ab3517)
This product has been referenced in:
- Kaeffer Bet al. Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells. PLoS One 6:e25562 (2011). ICC/IF; Human.Read more (PubMed: 22028779) »
- Andrews JLet al. CLOCK and BMAL1 regulate MyoD and are necessary for maintenance of skeletal muscle phenotype and function. Proc Natl Acad Sci U S A 107:19090-5 (2010). ChIP; Mouse.Read more (PubMed: 20956306) »
See all 5 publications for this product
Publishing research using ab3517? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"