with N-terminal added lysine conjugated to KLH, corresponding to amino acids 399-419 of Human HAT1.
The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500. Detects a band of approximately 46 kDa.
機能Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
配列類似性Belongs to the HAT1 family.
発生段階Highly expressed in mitotic cells (at protein level).
細胞内局在Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.