Anti-IPO5 抗体 (ab88695)
Key features and details
- Mouse polyclonal to IPO5
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IPO5 antibody
IPO5 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to IPO5 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein within Human IPO5. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH45640.1)
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ポジティブ・コントロール
- WB: Rat brain tissue lysate; HeLa cell lysate; Karyopherin (importin) beta 3 transfected 293T cell lysate ICC/IF: HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88695の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 124 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. -
配列類似性
Belongs to the importin beta family.
Contains 6 HEAT repeats.
Contains 1 importin N-terminal domain. -
細胞内局在
Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 3843 Human
- Entrez Gene: 306182 Rat
- Omim: 602008 Human
- SwissProt: O00410 Human
- Unigene: 712598 Human
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別名
- DKFZp686O1576 antibody
- FLJ43041 antibody
- IMB3 antibody
see all
画像
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Anti-IPO5 antibody (ab88695) at 1 µg/ml + rat brain tissue lysate at 50 µg
Predicted band size: 124 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Anti-IPO5 antibody (ab88695) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 124 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
All lanes : Anti-IPO5 antibody (ab88695) at 1 µg/ml
Lane 1 : Karyopherin (importin) beta 3 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 124 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted? -
ab88695, at 10 µg/ml, staining Karyopherin (importin) beta 3 in HeLa cell by immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab88695 は論文での使用が確認できていません。