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This antigen is present in serum on all immunoglobulins bearing kappa light chains. It is also present on approximately 50% of normal human B lymphocytes.
Measurement of kappa light chain in human lysed whole peripheral blood or mononuclear cells separated by density gradient. Studies of clonality of B cell lymphomas in tissue. Studies of clonality of B cell leukemias in peripheral blood. Kappa (FITC) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl of Kappa (FITC) is sufficient for labelling of 1x106 cells.
Our Abpromise guarantee covers the use of ab1185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
ab1185 has not yet been referenced specifically in any publications.