Anti-Kappa free light chains 抗体 [4C11] (ab1939)

製品の概要

  • 製品名Anti-Kappa free light chains antibody [4C11]
    Kappa free light chains 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4C11] to Kappa free light chains
  • 特異性Specific for  independent constantly expressed epitope of kappa chain. Reacts with free monomer and dimer of  kappa light chains but not with intact kappa bearing immunoglobulins.
  • アプリケーション適用あり: ELISA, WB, Flow Cyt, Othermore details
  • 種交差性
    交差種: Human
  • 免疫原

    Mixture of light chains of human immunoglobulin.

  • 特記事項Concentration varies from lot to lot and can be provided on request.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purity tested by electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名4C11
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Specific antibody titer is 1/200 000 by indirect ELISA
AP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
Flow Cyt Use 2µg for 106 cells.
Other Use at an assay dependent concentration.

ターゲット情報

  • 参照データベース
  • 別名
    • Free kappa light chain antibody
    • Free kappa light chains antibody
    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • Kappa free light chain antibody
    • Kappa light chain free antibody
    • Kappa light chains free antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Kappa free light chains antibody [4C11] 画像

  • Overlay histogram showing Ramos cells stained with ab1939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1939, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Kappa free light chains antibody [4C11] (ab1939) 使用論文

ab1939 has not yet been referenced specifically in any publications.

Product Wall

We only have information that ab1939 Kappa free light chains antibody [4C11] is specific for an independent constantly expressed epitope of kappa chain and that it reacts with free monomer and dimer of kappa light chains but not with intact kappa beari...

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I am sorry for the delay in getting back to you.

I now have further information to share with you in regards to the samples used with the Anti-Kappa free light chains antibody [4C11] (ab1939). As we have discussed previously, this antibody ha...

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Thank you for contacting us today.

I am sorry for the delay in getting back to you. As discussed over the phone, as you intend to use the antibodies ab1939, ab109489 and ab134083 in Western blotting and IHC, we would be able to offer you a te...

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Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

The protocolused for Western blotting was according to Towbin et al. in: PNAS, September 1, 1979, vol. 76, no. 9, 4350-435...

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Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab1939  has never been tested in ICC or IHC-P or IHC-Fr.  All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any ne...

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Thank you for your enquiry. Mab against free light chains of human Ig were selected on the basis of their reactivity with purified Bence-Jones proteins kappa- or lambda and absence of any reaction with whole molecules of IgA, IgG, IgM and IgE. Any...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"