Anti-Kallikrein 6 抗体 (ab109295)
Key features and details
- Goat polyclonal to Kallikrein 6
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Kallikrein 6 antibody
Kallikrein 6 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Kallikrein 6 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Cow -
免疫原
Synthetic peptide:
KEKPGVYTDVCTHIR
, corresponding to C terminal amino acids 228-242 of Mouse Kallikrein 6 (NP_035307.1) -
ポジティブ・コントロール
- Mouse Liver lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab109295 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. -
組織特異性
In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Inactivated by autolytic cleavage after Arg-80. -
細胞内局在
Secreted. Nucleus > nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress. - Information by UniProt
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参照データベース
- Entrez Gene: 19144 Mouse
- Entrez Gene: 29245 Rat
- SwissProt: P15947 Mouse
- SwissProt: P36374 Rat
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別名
- Bssp antibody
- hK 6 antibody
- hK6 antibody
see all
画像
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All lanes : Anti-Kallikrein 6 antibody (ab109295) at 2 µg/ml
Lane 1 : Mouse Liver lysate (in RIPA buffer) without immunizing peptide
Lane 2 : Mouse Liver lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109295 は論文での使用が確認できていません。