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Our Abpromise guarantee covers the use of ab64917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/50 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa).
In recent lot testing (Dec 2013), the apparent MW (130 kDa) is higher than the predicted MW (88 kDa) in over expression lysate, human brain and U251 cells under reduced and denatured conditions.
Flow cytometry analysis of NCI-H292 cells labeling K Cadherin with ab64917. A FITC-conjugated Goat anti-rabbit was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocarcinoma tissue sections labeling K Cadherin with ab64917.