Jurkat nuclear extract lysate (TPA + CI stimulated) (ab14850)

製品の概要

  • 製品名
    Jurkat nuclear extract lysate (TPA + CI stimulated)
    Jurkat ライゼート 製品一覧
  • 製品の詳細
    Jurkat nuclear extract lysate
  • 特記事項
    Cell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
    Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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  • 研究分野
  • 背景
    Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

参考文献

ab14850 has not yet been referenced specifically in any publications.

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