Jurkat nuclear extract lysate (PHA stimulated) (ab14848)

製品の概要

  • 製品名
    Jurkat nuclear extract lysate (PHA stimulated)
    Jurkat ライゼート 製品一覧
  • 製品の詳細
    Jurkat nuclear extract lysate
  • 特記事項
    Cell line: Jurkat (Human T cell lymphoblast-like).
    Cells were cultured in medium supplemented with 2 µg/ml PHA (Leucoagglutinin, PHA-L) for 2 days at 37°C immediately prior to harvesting.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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  • 研究分野
  • 背景
    Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

参考文献

ab14848 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry. I can confirm that this lysate has been tested in WB for NFATc1 activation but not for the other cytokines. I am able to provide details of the conditions of induction we used to prepare this extract : Cells were cul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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