The nuclear extract was prepared from the heat shock-treated Jurkat human T cell leukemia cell line (clone E6-1). Cells were cultured in medium heated at 45°C for 45 minutes immediately prior to harvesting.
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.