製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to JunD
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JunD.

    (Peptide available as ab74427.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Nuclear Lysates: HeLa, Jurkat

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能
    Transcription factor binding AP-1 sites.
  • 配列類似性
    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • Jun D antibody
    • jun D proto oncogene antibody
    • Jund antibody
    • JunD FL isoform antibody
    • JUND_HUMAN antibody
    • Transcription factor jun D antibody
    • Transcription factor jun-D antibody
    see all

画像

  • All lanes : Anti-JunD antibody (ab74428) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

ab74428 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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