Anti-Junctophilin-2 抗体 (ab79071)

製品の概要

  • 製品名
    Anti-Junctophilin-2 antibody
    Junctophilin-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Junctophilin-2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic 17 amino acid peptide near the carboxy terminus of human Junctophilin-2 (NP_065166)

  • ポジティブ・コントロール
    • Mouse brain tissue lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity chromatography purified via peptide column
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 74 kDa.Can be blocked with Junctophilin-2 peptide (ab110064).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic/sarcoplasmic reticulum. Contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.
  • 組織特異性
    Specifically expressed in skeletal muscle and heart.
  • 関連疾患
    Defects in JPH2 are the cause of cardiomyopathy familial hypertrophic type 17 (CMH17) [MIM:613873]. CMH17 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • 配列類似性
    Belongs to the junctophilin family.
    Contains 8 MORN repeats.
  • ドメイン
    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • 細胞内局在
    Cell membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ40969 antibody
    • JP-2 antibody
    • JP2 antibody
    • Jph2 antibody
    • JPH2_HUMAN antibody
    • Junctophilin 2 antibody
    • Junctophilin type 2 antibody
    • Junctophilin-2 antibody
    • OTTHUMP00000031651 antibody
    • OTTHUMP00000031652 antibody
    see all

Anti-Junctophilin-2 antibody 画像

  • Lane 1 : Anti-Junctophilin-2 antibody (ab79071) at 1 µg/ml
    Lane 2 : Anti-Junctophilin-2 antibody (ab79071) at 2 µg/ml

    Lane 1 : mouse brain tissue lysate
    Lane 2 : mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 64 kDa (possible isoform).

プロトコール

Anti-Junctophilin-2 antibody (ab79071) 使用論文

ab79071 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Sheep Tissue lysate - whole (Cardiac)
Specification
Cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録