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RabMAb

Anti-Junctional Adhesion Molecule 1 抗体 [EP1042Y] (ab52647)

製品の概要

  • 製品名
    Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y]
    Junctional Adhesion Molecule 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of human Junctional Adhesion Molecule 1.

  • ポジティブ・コントロール
    • Hela cell lysate and human liver tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/250.
  • 追加情報
    Is unsuitable for ICC.
  • ターゲット情報

    • 機能
      Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
    • 配列類似性
      Belongs to the immunoglobulin superfamily.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • 翻訳後修飾
      N-glycosylated.
    • 細胞内局在
      Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 321 antibody
      • CD321 antibody
      • CD321 antigen antibody
      • ESTM33 antibody
      • F11 receptor antibody
      • F11R antibody
      • JAM 1 antibody
      • JAM A antibody
      • JAM antibody
      • JAM-1 antibody
      • JAM-A antibody
      • JAM1 antibody
      • JAM1_HUMAN antibody
      • JAMA antibody
      • JCAM antibody
      • Jcam1 antibody
      • Junction adhesion molecule 1 antibody
      • Junction adhesion molecule, mouse, homolog of antibody
      • Junctional adhesion molecule 1 antibody
      • Junctional adhesion molecule A antibody
      • KAT antibody
      • Ly106 antibody
      • PAM 1 antibody
      • PAM-1 antibody
      • PAM1 antibody
      • Platelet adhesion molecule 1 antibody
      • Platelet adhesion molecule antibody
      • Platelet F11 receptor antibody
      • PRO301 antibody
      • UNQ264 antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
      Lane 2: Junctional Adhesion Molecule 1 knockout HAP1 whole cell lysate (40 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab52647 observed at 32 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab52647 was shown to specifically react with Junctional Adhesion Molecule 1 in wild-type HAP1 cells as signal was lost in Junctional Adhesion Molecule 1 knockout cells. Wild-type and Junctional Adhesion Molecule 1 knockout samples were subjected to SDS-PAGE. ab52647 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Junctional Adhesion Molecule 1 with Purified ab52647 at 1:100 dilution (6.15 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647) at 1/1000 dilution (purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 33 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647) at 1/2000 dilution (unpurified) + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa

    • Immunohistochemical staining of paraffin-embedded human liver using unpurified ab52647 at a 1/100 dilution.

    参考文献

    This product has been referenced in:
    • Brandt A  et al. Short-Term Intake of a Fructose-, Fat- and Cholesterol-Rich Diet Causes Hepatic Steatosis in Mice: Effect of Antibiotic Treatment. Nutrients 9:N/A (2017). Read more (PubMed: 28906444) »
    • Magara K  et al. Elevated expression of JAM-A promotes neoplastic properties of lung adenocarcinoma. Cancer Sci 108:2306-2314 (2017). IHC-P ; Human . Read more (PubMed: 28837251) »

    See all 5 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    投稿 Nov 18 2015

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue (mucosa and submucosa))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue (mucosa and submucosa)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    投稿 Oct 16 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    投稿 Oct 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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