Rabbit polyclonal to Josephin-2
Mouse, Rat, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (QLFSQEAADE ICKRLAPDSR LNPHRSLLGT GNYDVNVIMA ALQGLGLAAV) of Human Josephin-2 (NP_612207).
- Transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.
Contains 1 Josephin domain.
Information by UniProt
- FLJ29018 antibody
- JOS2_HUMAN antibody
- JOSD 2 antibody
Western blot - Josephin-2 antibody (ab87207)
Anti-Josephin-2 antibody (ab87207) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
21 kDaObserved band size:
22 kDa (why is the actual band size different from the predicted?
)Additional bands at:
28 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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