製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to JNK3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human JNK3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab101295, ab101295)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in U87mg whole cell lysate as well as the following tissue lysates: Human brain; Human spinal cord; Mouse hippocampus; Mouse cerebellum; Rat hippocampus; Rat cerebellum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).

ターゲット情報

  • 機能
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
  • 組織特異性
    Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • 関連疾患
    Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメイン
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾
    Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c Jun kinase 3 antibody
    • c-Jun N-terminal kinase 3 antibody
    • cJun N terminal kinase 3 antibody
    • FLJ12099 antibody
    • FLJ33785 antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • MAP kinase 10 antibody
    • MAP kinase antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • Mapk10 antibody
    • MGC50974 antibody
    • mitogen activated protein kinase 10 antibody
    • Mitogen-activated protein kinase 10 antibody
    • MK10_HUMAN antibody
    • p493F12 antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • protein kinase mitogen activated 10 antibody
    • SAPK1b antibody
    • Stress activated protein kinase 1b antibody
    • stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase JNK3 antibody
    see all

画像

  • All lanes : Anti-JNK3 antibody (ab87404) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Cerebellum Mouse Tissue Lysate
    Lane 5 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 6 : Rat Hippocampus Tissue Lysate
    Lane 7 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa,52 kDa. We are unsure as to the identity of these extra bands.The predicted molecular weight of MAPK10 is 52 kDa (SwissProt), however we expect to observe a banding pattern at 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

プロトコール

参考文献

ab87404 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing
Sample
Anopheles stephensi Cell lysate - whole cell (midgut)
Specification
midgut
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Apr 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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