Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) 抗体 (ab59196)

製品の概要

  • 製品名Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody
    JNK1+JNK2+JNK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to JNK1+JNK2+JNK3 (phospho T183 + Y185)
  • 特異性ab59196 detects endogenous levels of JNK1/2/3 only when phosphorylated at threonine183/tyrosine 185.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 183/tyrosine 185 (MMTPPYPVV)

  • ポジティブ・コントロール
    • 293 cell extract treated with UV

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 40, 60 kDa (predicted molecular weight: 48 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C Jun kinase 2 antibody
    • c Jun N terminal kinase 1 antibody
    • c Jun N terminal kinase 2 antibody
    • c Jun N terminal kinase 3 antibody
    • c-Jun N-terminal kinase 1 antibody
    • JNK 46 antibody
    • JNK 55 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1 antibody
    • JNK1A2 antibody
    • JNK2 antibody
    • JNK21B1/2 antibody
    • JNK2A antibody
    • JNK2ALPHA antibody
    • JNK2B antibody
    • JNK2BETA antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • Jun kinase antibody
    • JUN N terminal kinase antibody
    • MAP kinase 10 antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • MAPK 8 antibody
    • MAPK 9 antibody
    • MAPK10 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 10 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p493F12 antibody
    • p54a antibody
    • p54aSAPK antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • PRKM8 antibody
    • PRKM9 antibody
    • SAPK antibody
    • SAPK(beta) antibody
    • SAPK1 antibody
    • SAPK1a antibody
    • SAPK1b antibody
    • SAPK1c antibody
    • Stress activated protein kinase 1 antibody
    • Stress activated protein kinase 1a antibody
    • Stress activated protein kinase 1b antibody
    • Stress activated protein kinase 1c antibody
    • Stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK1 antibody
    • Stress activated protein kinase JNK2 antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase 1c antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody 画像

  • All lanes : Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody (ab59196) at 1/500 dilution

    Lane 1 : 293 cell extract treated with UV (5 mins)
    Lane 2 : 293 cell extract treated with UV (5 mins) with immunising phosphopeptide


    Predicted band size : 48 kDa
    Observed band size : 40 and 60 kDa (why is the actual band size different from the predicted?)

Anti-JNK1+JNK2+JNK3 (phospho T183 + Y185) antibody (ab59196) 使用論文

This product has been referenced in:
  • Taylor DM  et al. MAP kinase phosphatase 1 (MKP-1/DUSP1) is neuroprotective in Huntington's disease via additive effects of JNK and p38 inhibition. J Neurosci 33:2313-25 (2013). WB, IHC ; Mouse . Read more (PubMed: 23392662) »

See 1 Publication for this product

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