Anti-JNK1 + JNK2 + JNK3 (phospho T183 + Y185) 抗体 (ab59196)
Key features and details
- Rabbit polyclonal to JNK1 + JNK2 + JNK3 (phospho T183 + Y185)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-JNK1 + JNK2 + JNK3 (phospho T183 + Y185) antibody
JNK1 + JNK2 + JNK3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to JNK1 + JNK2 + JNK3 (phospho T183 + Y185) -
由来種
Rabbit -
特異性
ab59196 detects endogenous levels of JNK1/2/3 only when phosphorylated at threonine183/tyrosine 185. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human JNK1 + JNK2 + JNK3 aa 150-250 (phospho T183 + Y185). Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 183/tyrosine 185 (MMTPPYPVV) Swiss-Prot #: P45983/P45984/P53779
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 40, 60 kDa (predicted molecular weight: 48 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 40, 60 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5599 Human
- Entrez Gene: 5601 Human
- Entrez Gene: 5602 Human
- Entrez Gene: 116554 Rat
- Entrez Gene: 25272 Rat
- Entrez Gene: 50658 Rat
- Omim: 601158 Human
- Omim: 602896 Human
see all -
別名
- C Jun kinase 2 antibody
- c Jun N terminal kinase 1 antibody
- c Jun N terminal kinase 2 antibody
see all
画像
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All lanes : Anti-JNK1 + JNK2 + JNK3 (phospho T183 + Y185) antibody (ab59196) at 1/500 dilution
Lane 1 : 293 cell extract treated with UV (5 mins)
Lane 2 : 293 cell extract treated with UV (5 mins) with immunising phosphopeptide
Predicted band size: 48 kDa
Observed band size: 40,60 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab59196 は 2 報の論文で使用されています。
- Jiang ZC et al. Downregulated LRRK2 gene expression inhibits proliferation and migration while promoting the apoptosis of thyroid cancer cells by inhibiting activation of the JNK signaling pathway. Int J Oncol 55:21-34 (2019). PubMed: 31180559
- Taylor DM et al. MAP kinase phosphatase 1 (MKP-1/DUSP1) is neuroprotective in Huntington's disease via additive effects of JNK and p38 inhibition. J Neurosci 33:2313-25 (2013). WB, IHC ; Mouse . PubMed: 23392662