Anti-JNK1 (phospho T183 + Y185) 抗体 (ab46821)

製品の概要

• 製品名
  Anti-JNK1 (phospho T183 + Y185) antibody
  JNK1 一次抗体 製品一覧
• 製品の詳細
  Chicken polyclonal to JNK1 (phospho T183 + Y185)
• 特異性
  The region of JNK1 surrounding T183 + Y185 has a high degree of similarity to the corresponding regions in JNK2 and JNK3 and thus may cross react with these proteins if phosphorylated on the corresponding residues.
• アプリケーション
  適用あり: WB, ELISA, ICC/IFmore details
• 種交差性
  交差種: Mouse, Rat, Human
  
• 免疫原

  Synthetic peptide corresponding to Human JNK1 aa 180-188 (phospho T183 + Y185).
  Sequence:

  FMM(pT)P(pY)VVT

  Run BLAST with Run BLAST with
• ポジティブ･コントロール
  • PC12 cell extract.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• バッファー
  Preservative: None
  Constituents: PBS
• Concentration information loading...
• 精製度
  IgY fraction
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgY
• 研究分野
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • MAPK pathway

  • Immunology
  • Innate Immunity
  • TLR Signaling

関連製品

• Positive Controls
  • PC12 cytoplasmic extract lysate (ab14883)
  • PC12 nuclear extract lysate (ab14884)

アプリケーション

ターゲット情報

• 機能
  Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
• 配列類似性
  Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• ドメイン
  The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• 翻訳後修飾
  Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

• Target information above from: UniProt accession P45983 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 5599 Human
  • Entrez Gene: 26419 Mouse
  • Entrez Gene: 116554 Rat
  • Omim: 601158 Human
  • SwissProt: P45983 Human
  • SwissProt: Q91Y86 Mouse
  • SwissProt: P49185 Rat
  • Unigene: 138211 Human

  • Unigene: 21495 Mouse
  • Unigene: 4090 Rat

  see all
• 別名
  • AI849689 antibody
  • c Jun N terminal kinase 1 antibody
  • C-JUN kinase 1 antibody

  • c-Jun N-terminal kinase 1 antibody
  • EC 2.7.11.24 antibody
  • JNK 1 antibody
  • JNK antibody
  • JNK-46 antibody
  • JNK1A2 antibody
  • JNK21B1/2 antibody
  • MAP kinase 8 antibody
  • MAPK 8 antibody
  • mapk8 antibody
  • Mitogen activated protein kinase 8 antibody
  • Mitogen-activated protein kinase 8 antibody
  • MK08_HUMAN antibody
  • p54 gamma antibody
  • Prkm8 antibody
  • Protein kinase JNK1 antibody
  • Protein kinase, mitogen-activated, 8 antibody
  • SAPK 1 antibody
  • SAPK gamma antibody
  • SAPK1 antibody
  • Stress-activated protein kinase 1 antibody
  • Stress-activated protein kinase JNK1 antibody

  see all

プロトコール

• Immunocytochemistry & immunofluorescence protocols
• Western blot protocols