製品の概要

  • 製品名Anti-JNK1 (phospho T183) antibody
    JNK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to JNK1 (phospho T183)
  • 特異性The region of JNK1 surrounding T183 has a high degree of similarity to the corresponding regions in JNK2 and JNK3 and thus may cross react with these proteins if phosphorylated on the corresponding residues.
  • アプリケーション適用あり: WB, ICC/IF, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthesized phosphopeptide derived from human JNK1 around the phosphorylation site of threonine 183 (M-M-TP-P-Y)

  • ポジティブ・コントロール
    • Extracts from Hela cells and human breast carcinoma tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23226105
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI849689 antibody
    • c Jun N terminal kinase 1 antibody
    • C-JUN kinase 1 antibody
    • c-Jun N-terminal kinase 1 antibody
    • EC 2.7.11.24 antibody
    • JNK 1 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1A2 antibody
    • JNK21B1/2 antibody
    • MAP kinase 8 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p54 gamma antibody
    • Prkm8 antibody
    • Protein kinase JNK1 antibody
    • Protein kinase, mitogen-activated, 8 antibody
    • SAPK 1 antibody
    • SAPK gamma antibody
    • SAPK1 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Anti-JNK1 (phospho T183) antibody 画像

  • All lanes : Anti-JNK1 (phospho T183) antibody (ab47337)

    Lane 1 : Extract of Hela cells treated with Anisomycin (200ng/ml, 10min)
    Lane 2 : Extract of Hela cells, untreated


    Predicted band size : 48 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47337 at a 1/50 dilution.

    Left image : Un-treated

    Right image : Treated with P-peptide

Anti-JNK1 (phospho T183) antibody (ab47337) 使用論文

This product has been referenced in:
  • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). Rat . Read more (PubMed: 27301321) »
  • Ye S  et al. Thrombosis recanalization by paeoniflorin through the upregulation of urokinase-type plasminogen activator via the MAPK signaling pathway. Mol Med Rep 13:4593-8 (2016). WB ; Human . Read more (PubMed: 27082639) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"