JNK1 overexpression 293T lysate (whole cell) (ab94122)

製品の概要

  • 製品名JNK1 overexpression 293T lysate (whole cell)
  • 製品の詳細
    JNK1 overexpression lysate
  • 特記事項ab94122 is a 293T cell transfected lysate in which Human JNK1 has been transiently over-expressed using a pCMV-JNK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. Function: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

アプリケーション

Our Abpromise guarantee covers the use of ab94122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

JNK1 overexpression 293T lysate (whole cell) 画像

  • ab94122 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-JNK1 antibody (ab61190) at 1/500 dilution

    Lane 1 : JNK1 overexpression 293T lysate (whole cell) (ab94122)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

JNK1 overexpression 293T lysate (whole cell) (ab94122) 使用論文

ab94122 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"