遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-JNK1 抗体 [EPR140(2)] (ab110724)

製品の概要

  • 製品名
    Anti-JNK1 antibody [EPR140(2)]
    JNK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR140(2)] to JNK1
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human JNK1.

  • ポジティブ・コントロール
    • HeLa, 293T, K562, and MCF7 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能
      Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • 配列類似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • ドメイン
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻訳後修飾
      Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI849689 antibody
      • c Jun N terminal kinase 1 antibody
      • C-JUN kinase 1 antibody
      • c-Jun N-terminal kinase 1 antibody
      • EC 2.7.11.24 antibody
      • JNK 1 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1A2 antibody
      • JNK21B1/2 antibody
      • MAP kinase 8 antibody
      • MAPK 8 antibody
      • mapk8 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p54 gamma antibody
      • Prkm8 antibody
      • Protein kinase JNK1 antibody
      • Protein kinase, mitogen-activated, 8 antibody
      • SAPK 1 antibody
      • SAPK gamma antibody
      • SAPK1 antibody
      • Stress-activated protein kinase 1 antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1 antibody [EPR140(2)] 画像



    • Predicted band size : 48 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

      ab110724 was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa


    • Predicted band size : 48 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green).

      Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

      This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.

       

    プロトコール

    Anti-JNK1 antibody [EPR140(2)] (ab110724) 使用論文

    This product has been referenced in:
    • Kitanaka T  et al. JNK activation is essential for activation of MEK/ERK signaling in IL-1ß-induced COX-2 expression in synovial fibroblasts. Sci Rep 7:39914 (2017). Real Time PCR ; Cat . Read more (PubMed: 28054591) »
    • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). WB ; Rat . Read more (PubMed: 27301321) »

    See all 10 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cat Cell lysate - whole cell (synovial)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Treatment
    siRNA for scramble control, JNK1 and JNK2
    Specification
    synovial
    Blocking step
    (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    令 中野

    Verified customer

    投稿 Jun 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録