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Abcam's JNK Activity Screening Kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After washing to remove non-specifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.
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c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes.
|ATP (10 mM)||Yellow||1 x 50µl|
|c-Jun (1-79) Fusion Protein Beads||Red||1 x 800µl|
|Kinase Assay Buffer||WM||1 x 25ml|
|Kinase Extraction Buffer||NM||1 x 80ml|
|Phospho-cJun Specific Antibody||Green||1 x 50µl|
Our Abpromise guarantee covers the use of ab65784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|