製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to JMJD6
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 and 244 of Human JMJD6 (NP_055982).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Cal27, Raji or NIH3T3 cell lines.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/250.

ターゲット情報

  • 機能
    Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
  • 組織特異性
    Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
  • 配列類似性
    Belongs to the JMJD6 family.
    Contains 1 JmjC domain.
  • ドメイン
    The nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
  • 細胞内局在
    Nucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptotic cell clearance receptor antibody
    • Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
    • Histone arginine demethylase JMJD6 antibody
    • JmjC domain-containing protein 6 antibody
    • JMJD 6 antibody
    • JMJD6 antibody
    • JMJD6_HUMAN antibody
    • Jumonji domain containing 6 antibody
    • Jumonji domain-containing protein 6 antibody
    • KIAA0585 antibody
    • Lysyl-hydroxylase JMJD6 antibody
    • Peptide-lysine 5-dioxygenase JMJD6 antibody
    • Phosphatidylserine receptor antibody
    • Protein PTDSR antibody
    • PSR antibody
    • PTDSR 1 antibody
    • PTDSR antibody
    • PTDSR1 antibody
    see all

画像

  • Anti-JMJD6 antibody (ab96795) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 46 kDa
  • Immunohistochemistry of paraffin-embedded Cal27 xenograft using ab96795 at 1/100 dilution.
  • Anti-JMJD6 antibody (ab96795) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 46 kDa

参考文献

ab96795 has not yet been referenced specifically in any publications.

レビューと Q&A

We have found some reference data which indicates an apparent MW of 70 kD for JMJD6. Please see below: "The predicted 427-amino acid protein has 1 predicted transmembrane domain and an area in the C terminus that contains a series of serines ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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