製品の概要
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製品名Anti-JMJD6 antibody
JMJD6 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to JMJD6
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Zebrafish
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免疫原
A region within synthetic peptide: VLADSVDLQE STGIASDSSS DSSSSSSSSS SDSDSECESG SEGDGTVHRR, corresponding to C terminal amino acids 325-374 of Human JMJD6
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ポジティブ･コントロール
- Fetal thymus lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab50720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1.25 µg/ml. Predicted molecular weight: 46 kDa.Can be blocked with JMJD6 peptide (ab110802). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
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組織特異性Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
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配列類似性Belongs to the JMJD6 family.
Contains 1 JmjC domain. -
ドメインThe nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
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細胞内局在Nucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
- Information by UniProt
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参照データベース
- Entrez Gene: 508343 Cow
- Entrez Gene: 23210 Human
- Entrez Gene: 107817 Mouse
- Entrez Gene: 360665 Rat
- Entrez Gene: 266962 Zebrafish
- Omim: 604914 Human
- SwissProt: Q58DS6 Cow
- SwissProt: Q6NYC1 Human
see all -
別名
- Apoptotic cell clearance receptor antibody
- Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
- Histone arginine demethylase JMJD6 antibody
see all
画像
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Western blot - Anti-JMJD6 antibody (ab50720)Anti-JMJD6 antibody (ab50720) at 1.25 µg/ml + Fetal thymus lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Gel concentration 12%
プロトコール
参考文献
This product has been referenced in:
- Ruvolo PP et al. Role of MSC-derived galectin 3 in the AML microenvironment. Biochim Biophys Acta 1865:959-969 (2018). Read more (PubMed: 29655803) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"