製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to JMJD5
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • 免疫原

    Synthetic peptide within Human JMJD5 aa 350-399 (C terminal). The exact sequence is proprietary.
    Sequence:

    YPHDTHLLHNTSQVDVENPDLEKFPKFAKAPFLSCILSPGEILFIPVKYW


    Database link: Q8N371

  • ポジティブ・コントロール
    • Fetal liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Anti-JMJD5 antibody (ab28883) at 0.5 µg/ml + Human Fetal Liver Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (suggested dilution 1:50,000 - 100,000)

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab28883 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Caenorhabditis elegans Tissue lysate - nuclear (All body)
Specification
All body
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Pier Giorgio Amendola

Verified customer

投稿 Jun 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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