製品の概要

  • 製品名Anti-JM4 antibody
    JM4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to JM4
  • 特異性ab53113 detects endogenous levels of total JM4 protein.
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    A synthetic peptide derived from human JM4.

  • ポジティブ・コントロール
    • Human brain tissue and extracts from LOVO cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB 1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

ターゲット情報

  • 機能May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.
  • 組織特異性Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.
  • 配列類似性Belongs to the PRA1 family.
  • 細胞内局在Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Jena Muenchen 4 antibody
    • JM 4 antibody
    • PRA1 domain family member 2 antibody
    • PRA1 family protein 2 antibody
    • PRAF2 antibody
    • PRAF2_HUMAN antibody
    see all

Anti-JM4 antibody 画像

  • All lanes : Anti-JM4 antibody (ab53113) at 1/300 dilution

    Lane 1 : Mouse spleen whole tissue lysate
    Lane 2 : Mouse braint whole tissue lysate
    Lane 3 : Mouse heart whole tissue lysate
    Lane 4 : Mouse kidney whole tissue lysate
    Lane 5 : Mouse liver whole tissue lysate
    Lane 6 : Mouse embryonic fibroblasts lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa (possible non-specific binding).

    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-JM4 antibody (ab53113) at 1/300 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide


    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • ab53113 at 1/50 dilution staining JM4 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53113 at 1/50 dilution staining JM4 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Anti-JM4 antibody (ab53113) 使用論文

This product has been referenced in:
  • Chiu HC  et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . Read more (PubMed: 24671231) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12,5)
Sample Mouse Tissue lysate - whole (Spleen, Liver, Heart, Brain, kidney)
Specification Spleen, Liver, Heart, Brain, kidney
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2%
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投稿 Sep 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"