製品の概要

  • 製品名Anti-JIP2 antibody
    JIP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to JIP2
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebra finch
  • 免疫原

    A synthetic peptide derived from an internal of region human JIP2.

  • ポジティブ・コントロール
    • Human brain tissue and extracts from mouse brain cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50.

ターゲット情報

  • 機能The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins.
  • 組織特異性Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.
  • 配列類似性Belongs to the JIP scaffold family.
    Contains 1 PID domain.
    Contains 1 SH3 domain.
  • 細胞内局在Cytoplasm. Accumulates in cell surface projections.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C jun amino terminal kinase interacting protein 2 antibody
    • C-jun-amino-terminal kinase-interacting protein 2 antibody
    • Homologous to mouse JIP 1 antibody
    • IB 2 antibody
    • IB-2 antibody
    • IB2 antibody
    • Islet brain 2 antibody
    • Islet-brain-2 antibody
    • JIP 2 antibody
    • JIP-2 antibody
    • JIP2 antibody
    • JIP2_HUMAN antibody
    • JNK interacting protein 2 antibody
    • JNK MAP kinase scaffold protein 2 antibody
    • JNK MAP kinase scaffold protein JIP2 antibody
    • JNK-interacting protein 2 antibody
    • MAPK8IP2 antibody
    • Mitogen activated protein kinase 8 interacting protein 2 antibody
    • Mitogen-activated protein kinase 8-interacting protein 2 antibody
    • PRKM8 interacting protein like antibody
    • PRKM8IPL antibody
    see all

Anti-JIP2 antibody 画像

  • All lanes : Anti-JIP2 antibody (ab65211) at 1/500 dilution

    Lane 1 : Extracts from mouse brain cells
    Lane 2 : Extracts from mouse brain cells, plus immunising peptide


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab65211 staining the JIP2 (Red) in Zebra finch basal ganglia (striatum) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.3% Triton and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS Tween) for 24 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (Blue).

    See Abreview

  • ICC/IF image of ab65211 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65211, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-JIP2 antibody (ab65211) 使用論文

ab65211 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebra finch Tissue sections (basal ganglia (striatum)-fixed frozen sections)
Specification basal ganglia (striatum)-fixed frozen sections
Fixative Paraformaldehyde
Permeabilization Yes - TBS w/0.3% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"