Anti-Jarid2 抗体 - ChIP Grade (ab93288)

製品の概要

  • 製品名Anti-Jarid2 antibody - ChIP Grade
    Jarid2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Jarid2 - ChIP Grade
  • アプリケーション適用あり: WB, ELISA, ChIPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Partial recombinant fragment corresponding to amino acids 1130-1230 (LQLETSERRC QICQHLCYLS MVVQENENVV FCLECALRHV EKQKSCRGLK LMYRYDEEQI ISLVNQICGK VSGKNGSIEN CLSKPTPKRG PRKRATVDVP) of Human Jarid2 (NP_004964) with a proprietary tag.

  • ポジティブ・コントロール
    • 293 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2500. Predicted molecular weight: 139 kDa.
ELISA Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration.

ターゲット情報

  • 機能Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
  • 組織特異性During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
  • 配列類似性Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • ドメインThe ARID domain is required to target the PRC2 complex to its target genes.
    The GSGFP motif is required for the interaction with SUZ12.
  • 細胞内局在Nucleus. Colocalizes with the PRC2 complex on chromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • JARD2 antibody
    • JARD2_HUMAN antibody
    • JARID2 antibody
    • JMJ antibody
    • Jumonji AT rich interactive domain 2 antibody
    • Jumonji homolog antibody
    • Jumonji like protein antibody
    • Jumonji protein antibody
    • Jumonji/ARID domain containing protein 2 antibody
    • Jumonji/ARID domain-containing protein 2 antibody
    • Protein Jumonji antibody
    see all

Anti-Jarid2 antibody - ChIP Grade 画像

  • Anti-Jarid2 antibody - ChIP Grade (ab93288) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + 293 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 139 kDa
  • Anti-Jarid2 antibody - ChIP Grade (ab93288) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 139 kDa
    Observed band size : 37.11 kDa (why is the actual band size different from the predicted?)

Anti-Jarid2 antibody - ChIP Grade (ab93288) 使用論文

ab93288 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (HeLa)
Specification HeLa
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control Total Input DNA
Negative control Nonspecific IgG immunoprecipitation
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投稿 Nov 23 2011

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